BE2 Chromatography Unit aims to demonstrate the principles and practices of chromatography – an important operation for both small scale analysis and large scale production of biologics.
BE2 has two chromatography columns of variable bed height which are fed by a peristaltic pump. An injection point allows samples to be introduced onto the column. The unit has an on-line UV sensor for measuring protein concentration as it exits the column. BE2 is also equipped with a timer-controlled fraction collector. Size exclusion chromatography is the primary separation technique used for the demonstrations. Engineering principles such as the effect of feed flow rate and bed height on process performance are investigated.